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J Biosci Bioeng. 2011 Jan;111(1):22-5. doi: 10.1016/j.jbiosc.2010.08.020. Epub 2010 Sep 26.

Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures.

Author information

1
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University, Tempaku-ku, Nagoya 468-8502, Japan. ujita@meijo-u.ac.jp

Abstract

Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase.

PMID:
20870456
DOI:
10.1016/j.jbiosc.2010.08.020
[Indexed for MEDLINE]

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