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Proteomics. 2010 Jul;10(14):2728-32. doi: 10.1002/pmic.201000039.

Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches.

Author information

1
Department of Medicine, The Johns Hopkins University, Baltimore, MD 21224, USA.

Abstract

The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.

PMID:
20512790
PMCID:
PMC2934747
DOI:
10.1002/pmic.201000039
[Indexed for MEDLINE]
Free PMC Article

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