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Exp Hematol. 2010 Jan;38(1):46-54. doi: 10.1016/j.exphem.2009.11.001.

Clonal analysis and hierarchy of human bone marrow mesenchymal stem and progenitor cells.

Author information

1
Center of Excellence in Translational Human Stem Cell Research, University of California, Davis, Calif. 95616-8542, USA.

Abstract

OBJECTIVE:

This study was performed to assess adult human bone marrow mesenchymal stem/progenitor cells at a single-cell level and to determine a hierarchy based on proliferative potential.

MATERIALS AND METHODS:

Adult bone marrow mesenchymal cells expressing the enhanced green fluorescent protein (EGFP) were sorted as single cells into 24-well plates, each well confirmed with single EGFP-positive cells by fluorescence microscopy, and counted every 3 days. Colonies derived from single cells were expanded then sorted and evaluated using established differentiation protocols for adipogenic, chondrogenic, and osteogenic lineages. Cells were further analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR) (peroxisome proliferator-activated receptor[PPAR]-gamma2, LEP, LPL, LUM, COMP, BIG, RUNX2, IBSP, BGLAP) and immunocytochemistry (PPAR-gamma1/2, collagen II, bone sialoprotein II) specific for trilineage differentiation.

RESULTS:

Bone marrow mesenchymal cells were found to contain high proliferative potential (HPP) mesenchymal colony-forming cells (MCFC) (7%), low proliferative potential (LPP) MCFC (29%), mesenchymal cell clusters (MCC, 26%), and mature mesenchymal cells (MMC, 38%). All LPP-MCFC, MCC, and MMC colonies reached senescence at the end of the evaluation period. However, HPP-MCFC continued to grow, showed differentiation toward all three lineages, and demonstrated the capacity to give rise to secondary HPP-MCFC upon replating at a clonal level.

CONCLUSION:

These findings suggest that there is a low frequency of bone marrow-derived HPP-MCFC that can both self-renew at a single-cell level and differentiate toward multiple lineages of mesenchymal origin.

PMID:
19900502
PMCID:
PMC2807618
DOI:
10.1016/j.exphem.2009.11.001
[Indexed for MEDLINE]
Free PMC Article

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