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Methods Mol Biol. 2009;577:109-20. doi: 10.1007/978-1-60761-232-2_9.

Recombinant protein production by a Kaiko-baculovirus system.

Author information

1
Katakura Industries Co., Ltd., Saitama, Japan.

Abstract

Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the application of a highly efficient and reproducible baculovirus expression system with high throughput using Kaiko (silkworm).

PMID:
19718512
DOI:
10.1007/978-1-60761-232-2_9
[Indexed for MEDLINE]

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