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Anal Chem. 2009 Sep 1;81(17):7149-59. doi: 10.1021/ac900360b.

Nonprotein based enrichment method to analyze peptide cross-linking in protein complexes.

Author information

1
Department of Biochemistry, and Biodefense Proteomics Research Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA. fyan@aecom.yu.edu

Abstract

Cross-linking analysis of protein complexes and structures by tandem mass spectrometry (MS/MS) has advantages in speed, sensitivity, specificity, and the capability of handling complicated protein assemblies. However, detection and accurate assignment of the cross-linked peptides are often challenging due to their low abundance and complicated fragmentation behavior in collision-induced dissociation (CID). To simplify the MS analysis and improve the signal-to-noise ratio of the cross-linked peptides, we developed a novel peptide enrichment strategy that utilizes a cross-linker with a cryptic thiol group and using beads modified with a photocleavable cross-linker. The functional cross-linkers were designed to react with the primary amino groups in proteins. Human serum albumin was used as a model protein to detect intra- and intermolecular cross-linkages. Use of this protein-free selective retrieval method eliminates the contamination that can result from avidin-biotin based retrieval systems and simplifies data analysis. These features may make the method suitable to investigate protein-protein interactions in biological samples.

PMID:
19642656
PMCID:
PMC2765915
DOI:
10.1021/ac900360b
[Indexed for MEDLINE]
Free PMC Article

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