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Transplantation. 2009 Jul 15;88(1):38-41. doi: 10.1097/TP.0b013e3181a9ee6c.

Real-time bioluminescence imaging of macroencapsulated fibroblasts reveals allograft protection in rhesus monkeys (Macaca mulatta).

Author information

1
Center of Excellence in Translational Human Stem Cell Research, California National Primate Research Center, Davis, CA 95616-8542, USA. aftarantal@primate.ucdavis.edu

Abstract

BACKGROUND:

Encapsulation of cells has the potential to eliminate the need for immunosuppression for cellular transplantation. Recently, the TheraCyte device was shown to provide long-term immunoprotection of murine islets in a mouse model of diabetes. In this report, translational studies were undertaken using skin fibroblasts from an unrelated rhesus monkey donor that were transduced with an HIV-1-derived lentiviral vector expressing firefly luciferase permitting the use of bioluminescence imaging (BLI) to monitor cell survival over time and in a noninvasive manner.

METHODS:

Encapsulated cells were transplanted subcutaneously (n=2), or cells were injected without encapsulation (n=1) and outcomes compared. BLI was performed to monitor cell survival.

RESULTS:

The BLI signal from the encapsulated cells remained robust postinsertion and in one animal persisted for up to 1 year. In contrast, the control animal that received unencapsulated cells exhibited a complete loss of cell signal within 14 days.

CONCLUSIONS:

These data demonstrate that TheraCyte encapsulation of allogeneic cells provides robust immune protection in transplanted rhesus monkeys.

PMID:
19584678
PMCID:
PMC2744215
DOI:
10.1097/TP.0b013e3181a9ee6c
[Indexed for MEDLINE]
Free PMC Article

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