The haloprotease CPI produced by the moderately halophilic bacterium Pseudoalteromonas ruthenica is secreted by the type II secretion pathway

Cristina Sánchez-Porro; Encarnación Mellado; Anthony P Pugsley; Olivera Francetic; Antonio Ventosa

doi:10.1128/AEM.00156-09

The haloprotease CPI produced by the moderately halophilic bacterium Pseudoalteromonas ruthenica is secreted by the type II secretion pathway

Appl Environ Microbiol. 2009 Jun;75(12):4197-201. doi: 10.1128/AEM.00156-09. Epub 2009 Apr 17.

Authors

Cristina Sánchez-Porro¹, Encarnación Mellado, Anthony P Pugsley, Olivera Francetic, Antonio Ventosa

Affiliation

¹ Department of Microbiology and Parasitology, University of Seville, Spain.

Abstract

The gene (cpo) encoding the extracellular protease CPI produced by the moderately halophilic bacterium Pseudoalteromonas ruthenica CP76 was cloned, and its nucleotide sequence was analyzed. The cpo gene encodes a 733-residue protein showing sequence similarity to metalloproteases of the M4 family. The type II secretion apparatus was shown to be responsible for secretion of the haloprotease CPI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
DNA, Bacterial / genetics
Gene Order
Membrane Transport Proteins / metabolism
Metalloproteases / genetics
Metalloproteases / metabolism*
Molecular Sequence Data
Protein Transport
Pseudoalteromonas / enzymology*
Pseudoalteromonas / genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid

Substances

Bacterial Proteins
DNA, Bacterial
Membrane Transport Proteins
Metalloproteases

Associated data

GENBANK/FM863706
GENBANK/FM863707
GENBANK/FM863708