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Structure. 2008 Jun;16(6):965-75. doi: 10.1016/j.str.2008.03.010.

Solution structure of Alg13: the sugar donor subunit of a yeast N-acetylglucosamine transferase.

Author information

1
Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.

Abstract

The solution structure of Alg13, the glycosyl donor-binding domain of an important bipartite glycosyltransferase in the yeast Saccharomyces cerevisiae, is presented. This glycosyltransferase is unusual in that it is active only in the presence of a binding partner, Alg14. Alg13 is found to adopt a unique topology among glycosyltransferases. Rather than the conventional Rossmann fold found in all GT-B enzymes, the N-terminal half of the protein is a Rossmann-like fold with a mixed parallel and antiparallel beta sheet. The Rossmann fold of the C-terminal half of Alg13 is conserved. However, although conventional GT-B enzymes usually possess three helices at the C terminus, only two helices are present in Alg13. Titration of Alg13 with both UDP-GlcNAc, the native glycosyl donor, and a paramagnetic mimic, UDP-TEMPO, shows that the interaction of Alg13 with the sugar donor is primarily through the residues in the C-terminal half of the protein.

PMID:
18547528
PMCID:
PMC2486831
DOI:
10.1016/j.str.2008.03.010
[Indexed for MEDLINE]
Free PMC Article

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