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PLoS One. 2008 Apr 30;3(4):e2061. doi: 10.1371/journal.pone.0002061.

Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.

Author information

1
Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America.

Abstract

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.

PMID:
18446240
PMCID:
PMC2329591
DOI:
10.1371/journal.pone.0002061
[Indexed for MEDLINE]
Free PMC Article

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