Objective: To explore the relationship between A-922G genetic polymorphism in the 5' flanking region of the endothelial nitric oxide synthase (eNOS) gene and ischemic stroke.
Methods: A 1:1 matched case-control study was conducted. 309 patients suffered from ischemic stroke were selected from two general hospitals in Shenzhen. The controls were selected by the same gender and ethnic group, and each pair's ages were permitted of differ within 5 years. eNOS A-922G genotypes were determined by using the real-time PCR technology for SNP genotyping with Taqman MGB probes. The cases and controls were interviewed by using the same questionnaire. Results The genotype frequencies (AA, AG and GG) of eNOS A-922G were 78.96%, 17.80, and 3.24% in patients, and 85.11%, 13.59, and 1.29% in controls. The allele frequencies of eNOS--922G in case group (12.14%) were higher than those in the controls (8.09%) (P = 0.018). OR value of ischemic stroke for subjects with at least one G allele was 1.523 (OR 95% CI 1.005-2.309). Results of conditional logistic regression demonstrated that eNOS A-922G genetic polymorphism also had significant effects on ischemic stroke (OR = 2.156, 95% CI 1.081-4.299) after adjusting smoking, alcohol drinking, waist-to-hipratio, body mass index and history of hypertension, et al.
Conclusion: It was suggested that A-922G gene polymorphism of eNOS gene could associate with the higher risk of ischemic stroke susceptibility.