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Curr Protoc Mol Biol. 2007 Jul;Chapter 21:Unit 21.13. doi: 10.1002/0471142727.mb2113s79.

ChIP-chip for genome-wide analysis of protein binding in mammalian cells.

Author information

1
Yale University School of Medicine, New Haven, Connecticut, USA.

Abstract

ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the genome. UNIT 21.9 describes a protocol for analysis of protein-DNA interactions in yeast cells. This unit introduces an alternative protocol developed for mammalian cells.

PMID:
18265397
DOI:
10.1002/0471142727.mb2113s79
[Indexed for MEDLINE]

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