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Curr Protoc Mol Biol. 2005 Nov;Chapter 26:Unit 26.6. doi: 10.1002/0471142727.mb2606s72.

RNAi in transgenic plants.

Author information

1
Iowa State University, Ames, Iowa, USA.

Abstract

RNA silencing in plants is a rapid and facile approach for assignment of gene function and virus resistance. It allows selective RNA degradation through a mechanism that is given specificity by short interfering RNAs. The RNA silencer sequences are normally delivered as transgenes or a part of virus-vectors. This unit provides Basic Protocols for transgene and virus induced silencing. It also includes a Basic Protocol for protein overexpression using viral proteins that suppress silencing.

PMID:
18265365
DOI:
10.1002/0471142727.mb2606s72
[Indexed for MEDLINE]

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