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Fertil Steril. 2008 Oct;90(4):1109-18. Epub 2008 Feb 20.

Identification of human spermatogenesis-related proteins by comparative proteomic analysis: a preliminary study.

Author information

1
Department of Histology and Embryology, Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.

Abstract

OBJECTIVE:

To identify human spermatogenesis-related proteins.

DESIGN:

Prospective study.

SETTING:

University research laboratory.

PATIENT(S):

Three fertile men with normal spermatogenesis, 3 azoospermic patients with sloughing and disorganization of germ cells.

INTERVENTION(S):

Testicular tissue samples were collected by biopsy after informed consent.

MAIN OUTCOME MEASURE(S):

The protein expressional profiles of human testes of fertile men and azoospermic patients were compared using a proteomic approach by combining two-dimensional gel electrophoresis analyses and mass spectrometry. Bioinformatic analysis helped to reveal the regulation pathway. Expression of some selected proteins in normal and pathological testes was analyzed by immunohistochemistry.

RESULT(S):

Ten protein spots were identified as expressing differentially between the normal testes and pathological testes with sloughing and disorganization of germ cells; these included the phospholipid hydroperoxide glutathione peroxidase, peroxiredoxin 4 (Prx4), heat shock protein beta-1 (HSP27), and cathepsin D (CTSD). Bioinformatic analysis revealed that many differentially expressed proteins participate in cellular proliferation, apoptosis, and cell death and helped us to focus on a few of them. Immunohistochemical analysis of Prx4, HSP27, and CTSD confirmed the results obtained by proteomic analysis.

CONCLUSION(S):

These 10 proteins may help in elucidating the pathways involved in human spermatogenesis.

[Indexed for MEDLINE]

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