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Methods Mol Med. 2007;131:47-61.

In vitro methods to study RNA interference during an adenovirus infection.

Abstract

RNA interference (RNAi) has attracted a lot of interest during recent years as a method to knock-down gene expression and as a possible antiviral system. Here we present a collection of in vitro methods to study RNAi and the effect of an adenovirus infection on RNAi. We describe methods to measure the two key enzymatic complexes involved in RNAi: Dicer and RISC.

PMID:
17656774
DOI:
10.1007/978-1-59745-277-9_4
[Indexed for MEDLINE]

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