c-src structure in human cancers with elevated pp60c-src activity

P Wang; F Fromowitz; M Koslow; N Hagag; B Johnson; M Viola

doi:10.1038/bjc.1991.344

c-src structure in human cancers with elevated pp60c-src activity

Br J Cancer. 1991 Sep;64(3):531-3. doi: 10.1038/bjc.1991.344.

Authors

P Wang¹, F Fromowitz, M Koslow, N Hagag, B Johnson, M Viola

Affiliation

¹ Department of Medicine, State University of New York, Stony Brook 11794.

Abstract

We used RNAase protection and restriction fragment length polymorphism assays to detect activating mutations of c-src in a spectrum of human tumours. No mutations were detected at codons 98, 381, 444, and 530. We conclude that mutational activation is not the mechanism of enhancement of pp60c-src-specific kinase activity found in a number of human cancer types.

MeSH terms

Base Sequence
Codon
Genes, src*
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation*
Neoplasms / enzymology
Neoplasms / genetics*
Oligodeoxyribonucleotides
Polymorphism, Restriction Fragment Length
Protein-Tyrosine Kinases / genetics
Protein-Tyrosine Kinases / metabolism
Proto-Oncogene Proteins pp60(c-src) / genetics
Proto-Oncogene Proteins pp60(c-src) / metabolism*
RNA, Messenger / genetics

Substances

Codon
Oligodeoxyribonucleotides
RNA, Messenger
Protein-Tyrosine Kinases
Proto-Oncogene Proteins pp60(c-src)