Biodegradation of chlorferon and diethylthiophosphate by consortia enriched from waste cattle dip solution

Bioresour Technol. 2007 Jul;98(10):1916-23. doi: 10.1016/j.biortech.2006.07.038. Epub 2006 Sep 20.

Abstract

Chlorferon and diethylthiophosphate (DETP) are the hydrolysis products of coumaphos, an organophosphate pesticide. In this research, two consortia of bacterial cultures, one responsible for degrading chlorferon and the other for degrading DETP, were selectively enriched from waste cattle dip solution. The enriched cultures were used as inocula to grow biomass for biodegradation studies. For chlorferon degradation, the optimum biomass concentration was found to be 80g/L, and pH 7.5 was selected as the optimal operating pH. Chlorferon degradation was characterized by substrate inhibition kinetics with parameter values estimated to be V(m)=0.062+/-0.011mg/(g-biomass)h, K(m)=21+/-7mg/L, and K(Si)=118+/-45mg/L. For DETP degradation, the optimum biomass concentration was found to be 60g/L, and the optimum pH was in the range of 7.5-8. DETP degradation was characterized by Michaelis-Menten kinetics with parameter values estimated to be V(m)=1.52+/-0.10mg/(g-biomass)h and K(m)=610+/-106mg/L.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / isolation & purification
  • Bacteria / metabolism*
  • Biodegradation, Environmental
  • Biomass
  • Cattle
  • Hydrolysis
  • Kinetics
  • Pesticides / chemistry
  • Pesticides / metabolism*
  • Phosphates / isolation & purification
  • Phosphates / metabolism*
  • Solutions / chemistry
  • Umbelliferones / isolation & purification
  • Umbelliferones / metabolism*
  • Waste Disposal, Fluid
  • Waste Products / analysis

Substances

  • Pesticides
  • Phosphates
  • Solutions
  • Umbelliferones
  • Waste Products
  • chlorferron
  • thiophosphoric acid