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Biochem Biophys Res Commun. 1990 Jul 31;170(2):569-75.

Survey of oncogene and growth factor/receptor gene expression in cancer cells by intron-differential RNA/PCR.

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1
Department of Obstetrics and Gynecology, University of Minnesota, Minneapolis 55455.

Erratum in

  • Biochem Biophys Res Commun 1990 Nov 15;172(3):1407.

Abstract

To elucidate the possible roles of proto-oncogenes and growth factors in estrogen-regulated cell proliferation of human breast and gynecologic cancers, we have determined the gene expressions of c-myc, transforming growth factor-alpha and beta 1 (TGF-alpha, beta 1) and epidermal growth factor receptor (EGFR) in a number of these cancer cell lines by using an intron-Differential (ID) RNA/PCR method, which differentially identifies the amplified cDNA from PCR products of genomic DNA contaminants. With this method, we demonstrated the expression of these genes, except EGFR, in an estrogen-dependent breast cancer cell line (CAMA-1). Our results show that TGF-alpha/EGF does not function as an autocrine factor in this cell line. Accordingly, it is unlikely that the TGF-alpha/EGFR system participates as a mediator in the estrogen-induced cell proliferation of CAMA-1 cells. The ID RNA/PCR method is a rapid, sensitive and specific technique for mRNA phenotyping and will have great clinical utility.

PMID:
1696473
DOI:
10.1016/0006-291x(90)92129-n
[Indexed for MEDLINE]

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