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Appl Environ Microbiol. 2005 Dec;71(12):8974-7.

Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli.

Author information

1
Department of Technical Chemistry and Biotechnology, Institute of Chemistry and Biochemistry, Greifswald University, Soldmannstr. 16, D-17487 Greifswald, Germany.

Abstract

To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.

PMID:
16332903
PMCID:
PMC1317389
DOI:
10.1128/AEM.71.12.8974-8977.2005
[Indexed for MEDLINE]
Free PMC Article

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