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Anal Chem. 2005 Oct 15;77(20):6609-17.

Disposable hydrophobic surface on MALDI targets for enhancing MS and MS/MS data of peptides.

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Department of Pharmacology and Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.


Automated analyses in MALDI MS are complicated by the uneven distribution of analyte over the sample spot, resulting in areas of analyte localization, or "sweet spots". Hence, the ability to concentrate and localize samples is advantageous, especially for automated studies involving low concentrations of analyte. A method for rapidly creating a removable and affordable hydrophobic surface that is free from memory effect is presented. The potential for such compounds to serve as a practical coating for MALDI targets is examined. An example compound with a complete methodology is shown to increase sample homogeneity, peak intensity, and resolution when used for peptide mixtures with CHCA and DHB.

[Indexed for MEDLINE]

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