Comparison of the quantitative formalin ethyl acetate concentration technique and agar plate culture for diagnosis of human strongyloidiasis

P M Intapan; W Maleewong; T Wongsaroj; S Singthong; N Morakote

doi:10.1128/JCM.43.4.1932-1933.2005

Comparison of the quantitative formalin ethyl acetate concentration technique and agar plate culture for diagnosis of human strongyloidiasis

J Clin Microbiol. 2005 Apr;43(4):1932-3. doi: 10.1128/JCM.43.4.1932-1933.2005.

Authors

P M Intapan¹, W Maleewong, T Wongsaroj, S Singthong, N Morakote

Affiliation

¹ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. pewpan@kku.ac.th

Abstract

The quantitative formalin ethyl acetate concentration technique (QFEC) was compared to agar plate culture (APC) for the detection of Strongyloides stercoralis larvae. QFEC could substitute for APC only when the parasite load was higher than 50 larvae per g of stool. This study serves as a good reminder to those conducting stool exams about the sensitivity and specificity limitations of both techniques.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Acetates
Agar
Animals
Centrifugation / methods
Feces / parasitology
Formaldehyde
Humans
Intestinal Diseases, Parasitic / diagnosis*
Intestinal Diseases, Parasitic / parasitology
Larva
Parasitology / methods*
Strongyloides stercoralis / growth & development
Strongyloides stercoralis / isolation & purification*
Strongyloidiasis / diagnosis*
Strongyloidiasis / parasitology

Substances

Acetates
Formaldehyde
ethyl acetate
Agar