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Rev Med Chil. 2004 Dec;132(12):1475-82.

[P450Arom and estrogenic microenvironment of eutopic endometria in endometriosis].

[Article in Spanish]

Author information

1
Instituto de Investigaciones Materno-Infantil, Hospital Clínico San Borja Arriarán, Facultad de Medicina, Universidad de Chile. cjohnson@med.uchile.cl

Abstract

BACKGROUND:

Endometriosis, a common gynecologic disorder characterized by endometrial glands and stroma outside the uterus, is diagnosed by direct visualization of peritoneal and ovarian implants during laparoscopy.

AIM:

To study the estrogenic microenvironment in eutopic endometria of women with and without endometriosis.

PATIENTS AND METHODS:

Eutopic endometria, obtained during laparoscopy from 23 women with endometriosis and 20 fertile cyclic women undergoing tubal sterilization, was studied. P450Arom mRNA expression (RT-PCR) was measured. Also, P450Arom activity was assessed measuring testosterone conversion to estradiol and the concentration of this last hormone in cultured endometrial explants.

RESULTS:

Age and body mass index was similar in both groups studied. Seventy nine percent of endometria from women with endometriosis and in 29.4% from control group expressed P450Arom mRNA (p <0.01). The intensity of the band was higher in secretory endometria from women with endometriosis when compared to controls (p <0.01), but it was similar during the proliferative phase. Estradiol secretion to the culture media by proliferative endometria explants from women with endometriosis was 3-fold higher than secretory endometria (p <0.01) and endometria from control women in both phases. P450Arom activity, in the presence of testosterone, was 7-fold higher in endometrial cultures from women with endometriosis, when compare with the basal culture (p <0.01). However, in endometrial explant cultures from control women, this activity was not statistical different.

CONCLUSIONS:

These results indicate that in women with endometriosis, the microenvironment in the endometria is estrogenic as a consequence of an increased expression and activity of the P450Arom.

PMID:
15743158
[Indexed for MEDLINE]
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