Send to

Choose Destination
Inflamm Res. 2003 Apr;52(4):177-84.

Effect of lipopolysaccharide on D-fructose transport across rabbit jejunum.

Author information

Physiology Unit, Dept. of Pharmacology and Physiology, Veterinary Faculty, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.



To investigate alterations in the transport of D-fructose across the rabbit jejunum when the gut is exposed in vitro to lipopolysaccharide (LPS), an endotoxin causative agent of sepsis.


D-fructose intestinal transport was assesed employing three techniques: sugar uptake measurements in rings of everted jejunum (micromol/D-fructose/ml cell water), transepithelial flux measurements in Ussing-type chambers (micromol D-fructose/cm2/h) and transport assays in preparation of brush border membrane vesicles (pmoles D-fructose/mg protein). Samples were taken from the bathing solution and from the extracts of the tissue for radioactivity counting.


Adding LPS (3 microg/ml) to tissue decreased the uptake and mucosal to serosal flux of 5 mM D-fructose across the enterocyte. LPS did not modify sugar uptake across brush border membrane vesicles. The inhibitory effect of LPS was suppressed by W-13 (5 x 10(-6) M), a Ca-calmodulin antagonist, and staurosporine (10(-7) and 10(-6) M) and GF-109203X (10(-6) M) a nonselective and selective protein kinase C (PKC) inhibitor respectively. Tumor Necrosis Factor (TNF-alpha), an immunoregulatory cytokine involved in septic responses occurring during bacterial infection at concentrations 3 x 10(-4) to 3 microg/ml, did not affect the sugar transport.


LPS can inhibit the intestinal uptake of D-fructose across the rabbit jejunum in vitro by intracellular processes related to PKC and calmodulin protein.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center