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J Am Chem Soc. 2003 Jan 15;125(2):344-5.

DNA detection and signal amplification via an engineered allosteric enzyme.

Author information

1
Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA.

Abstract

Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 min under physiological conditions.

PMID:
12517141
PMCID:
PMC2453066
DOI:
10.1021/ja027885u
[Indexed for MEDLINE]
Free PMC Article

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