Objective: To investigate the roles of microfilaments in the expression of collagenase and TIMP-1 in normal and hypertrophic scar.
Methods: Cell culture and Northern blot hybridization methods were used in this study.
Results: After the disruption of microfilaments with cytochalasin B, mRNA contents of collagenase and TIMP-1 both increased significantly in normal and hypertrophic scar fibroblasts.
Conclusion: The result suggest that the microfilament cytoskeleton may regulate the synthesis and degradation of ECM, which may be a mechanism of gene regulation during wound healing.