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Appl Environ Microbiol. 2002 Jul;68(7):3651-4.

Cloning of a gene encoding raw-starch-digesting amylase from a Cytophaga sp. and its expression in Escherichia coli.

Author information

1
Department of Food Science, National Chung Hsing University, 250 Kuokang Road, Taichung 402, Taiwan, Republic of China. cljeang@nchu.edu.tw

Abstract

A raw-starch-digesting amylase (RSDA) gene from a Cytophaga sp. was cloned and sequenced. The predicted protein product contained 519 amino acids and had high amino acid identity to alpha-amylases from three Bacillus species. Only one of the Bacillus alpha-amylases has raw-starch-digesting capability, however. The RSDA, expressed in Escherichia coli, had properties similar to those of the enzyme purified from the Cytophaga sp.

PMID:
12089056
PMCID:
PMC126781
DOI:
10.1128/aem.68.7.3651-3654.2002
[Indexed for MEDLINE]
Free PMC Article

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