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Invest Ophthalmol Vis Sci. 2002 Jan;43(1):267-73.

Phenotypic variation of retinal pigment epithelium in age-related macular degeneration.

Author information

1
Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, Alabama 35294-0009, USA.

Abstract

PURPOSE:

To determine whether retinal pigment epithelium (RPE) in eyes with age-related macular degeneration (ARMD) express vimentin and alpha smooth muscle actin (alphaSMA), two cytoskeletal proteins associated with phenotypic variation in culture.

METHODS:

Six eyes with late ARMD and three age-matched control eyes were preserved in buffered 4% paraformaldehyde and cryosectioned at 10 microm. Stages of RPE morphology and pigmentation were assessed by the Alabama Age-Related Macular Degeneration Grading System. Vimentin, alphaSMA, and glial fibrillary acidic protein (GFAP) expression was detected by indirect immunofluorescence. These results were compared with regional variations in disease severity.

RESULTS:

RPE changes in ARMD included acquired expression of vimentin, but alphaSMA-positive cells were rare. GFAP expression increased in Müller cells in the neural retina in association with RPE changes and photoreceptor degeneration.

CONCLUSIONS:

The initial stages of RPE changes in eyes with ARMD mimic those reported for cultured RPE cells. The absence of alphaSMA-positive cells in regions of RPE atrophy suggests that RPE are lost rather than persist in a dedifferentiated state.

PMID:
11773041
[Indexed for MEDLINE]

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