Diagnosis of Oropouche virus infection by RT-nested-PCR

J Med Virol. 2002 Jan;66(1):139-42. doi: 10.1002/jmv.2122.

Abstract

Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Bunyaviridae Infections / diagnosis*
  • DNA Primers
  • DNA, Viral / analysis
  • Humans
  • Molecular Sequence Data
  • RNA, Viral / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Sequence Analysis, DNA
  • Simbu virus / genetics
  • Simbu virus / isolation & purification*

Substances

  • DNA Primers
  • DNA, Viral
  • RNA, Viral