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J Clin Microbiol. 2001 Oct;39(10):3709-11.

Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110.

Author information

1
Division of TB Control, Refugee and Migrant Health, University of Maryland-Baltimore, Baltimore, Maryland 21201, USAcroninw@dhmh.state.md.us.

Abstract

Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (< or =5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P < 0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.

PMID:
11574598
PMCID:
PMC88414
DOI:
10.1128/JCM.39.10.3709-3711.2001
[Indexed for MEDLINE]
Free PMC Article

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