NPDC-1, a regulator of neural cell proliferation and differentiation, interacts with E2F-1, reduces its binding to DNA and modulates its transcriptional activity

I Sansal; E Dupont; D Toru; C Evrard; P Rouget

doi:10.1038/sj.onc.1203843

NPDC-1, a regulator of neural cell proliferation and differentiation, interacts with E2F-1, reduces its binding to DNA and modulates its transcriptional activity

Oncogene. 2000 Oct 12;19(43):5000-9. doi: 10.1038/sj.onc.1203843.

Authors

I Sansal¹, E Dupont, D Toru, C Evrard, P Rouget

Affiliation

¹ Unité de Génétique Oncologique, CNRS-UMR-1599, Institut Gustave Roussy, PR-1, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France.

PMID: 11042687
DOI: 10.1038/sj.onc.1203843

Abstract

We have previously identified NPDC-1, a neural factor involved in the control of proliferation and differentiation, and we have shown that the stable introduction of NPDC-1 into transformed cells down-regulates cell proliferation both by increasing the generation time and by suppressing transformed properties. The data presented here indicate that, in vitro, NPDC-1 is able to interact with the transcription factor E2F-1 and some cell cycle proteins, such as D-cyclins and cdk2. In addition, two-hybrid experiments in mammalian cells show that the interaction between NPDC-1 and E2F-1 can also occur in vivo. This interaction reduces the binding of E2F-1 to DNA and its transcriptional activity. Taken together, the data suggest that NPDC-1 could influence cell cycle progression and neural differentiation through its association with E2F-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Binding Sites
Carrier Proteins*
Cell Cycle Proteins / metabolism
DNA / antagonists & inhibitors
DNA / genetics
DNA / metabolism*
DNA-Binding Proteins / antagonists & inhibitors
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
E2F Transcription Factors
E2F1 Transcription Factor
Genes, Reporter / genetics
Glutathione Transferase / genetics
Humans
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Nerve Tissue Proteins / physiology*
Peptide Mapping
Promoter Regions, Genetic
Rabbits
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / pharmacology
Retinoblastoma-Binding Protein 1
Transcription Factor DP1
Transcription Factors / antagonists & inhibitors
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcription, Genetic / drug effects
Transcription, Genetic / physiology*
Transfection

Substances

Carrier Proteins
Cell Cycle Proteins
DNA-Binding Proteins
E2F Transcription Factors
E2F1 Transcription Factor
E2F1 protein, human
NPDC1 protein, human
Nerve Tissue Proteins
Recombinant Fusion Proteins
Retinoblastoma-Binding Protein 1
Transcription Factor DP1
Transcription Factors
DNA
Glutathione Transferase