Objective: To examine the obesity candidate genes glucagon-like-peptide receptor (GLP1R), agouti signaling protein (ASIP) and the melanocortin receptors 4 and 5 (MC4R and MC5R) for DNA polymorphisms in their coding regions.
Subjects: Unrelated, non-diabetic Pima Indians (8 to 12 from each extreme of body fat).
Measurements: DNA sequencing within the coding regions of each gene.
Result: Only one variant was detected, a silent substitution in exon 6 of GLP1R.
Conclusion: The exclusion of any common amino-acid polymorphisms (allele frequency > or = 0.20). implies that structural variants of these genes do not contribute to variation in the high level of obesity observed among the Pima Indians.