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J Neurogastroenterol Motil. 2015 Oct 1;21(4):552-9. doi: 10.5056/jnm15079.

Surgical Intervention to Rescue Hirschsprung Disease in a Rat Model.

Author information

1
Department of Anatomy and Neuroscience, University of Melbourne, Parkville, VIC, Australia.
2
Department of Pediatric Surgery and Pediatric Urology, University Children's Hospital Tuebingen, Tuebingen, Germany.
3
Eccles Institute of Neuroscience, John Curtin School of Medical Research and Medical School, Australian National University, Canberra, ACT, Australia.
4
Department of Pediatrics and Child Health, Canberra Hospital, Canberra, Australia.

Abstract

BACKGROUND/AIMS:

Rats with a spontaneous null mutation in endothelin receptor type B or Ednrb (sl/sl; spotting lethal) lack enteric neurons in the distal bowel and usually die within the first week after birth. This early postnatal lethality limits their use for examining the potential of cell therapy to treat Hirschsprung disease, and for studies of the influence of EDNRB on the mature CNS and vascular systems.

METHODS:

We have developed a surgical intervention to prolong the life of the spotting lethal sl/sl rat, in which we perform a colostomy on postnatal (P) day 4-6 rats to avoid the fatal obstruction caused by the lack of colonic enteric neurons.

RESULTS:

The stomas remained patent and functional and the rats matured normally following surgery. Weight gains were comparable between control and Hirschsprung phenotype (sl/sl) rats, which were followed until 4 weeks after surgery (5 weeks old). We confirmed the absence of enteric neurons in the distal colon of rats whose lives were saved by the surgical intervention.

CONCLUSIONS:

This study provides a novel approach for studying EDNRB signalling in multiple organ systems in mature rats, including an animal model to study the efficacy of cell therapy to treat Hirschsprung disease.

KEYWORDS:

Colostomy; Endothelin signalling; Enteric nervous system; Hirschsprung disease

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