Send to

Choose Destination
PLoS One. 2017 Aug 14;12(8):e0183136. doi: 10.1371/journal.pone.0183136. eCollection 2017.

Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.

Author information

Aix Marseille Univ, INSERM, GMGF, Marseille, France.
APHM, Hôpital la Timone, Service de Biologie Cellulaire, Marseille, France.
APHM, Hôpital Nord, Department of Thoracic Oncology-Pleural diseases-Interventional pulmonology, Marseille, France.
APHM, Hôpital la Timone, Département de Génétique Médicale et Centre de Ressources Biologiques, Marseille, France.
APHM, Hôpital la Timone, Service de Pharmacologie Clinique & Centre d'Investigation Clinique-CPCET, Marseille, France.
Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France.
Aix Marseille Univ, APHM, Marseille Early Phases Cancer Trials Center CLIP2, Marseille, France.
Aix Marseille Univ, Marseille, France.


The type V intermediate filament lamins are the principal components of the nuclear matrix, including the nuclear lamina. Lamins are divided into A-type and B-type, which are encoded by three genes, LMNA, LMNB1, and LMNB2. The alternative splicing of LMNA produces two major A-type lamins, lamin A and lamin C. Previous studies have suggested that lamins are involved in cancer development and progression. A-type lamins have been proposed as biomarkers for cancer diagnosis, prognosis, and/or follow-up. The aim of the present study was to investigate lamins in cancer cells from metastatic pleural effusions using immunofluorescence, western blotting, and flow cytometry. In a sub-group of lung adenocarcinomas, we found reduced expression of lamin A but not of lamin C. The reduction in lamin A expression was correlated with the loss of epithelial membrane antigen (EMA)/MUC-1, an epithelial marker that is involved in the epithelial to mesenchymal transition (EMT). Finally, the lamin A expression was inversely correlated with the number of metastatic sites and the WHO Performance status, and association of pleural, bone and lung metastatic localizations was more frequent when lamin A expression was reduced. In conclusion, low lamin A but not lamin C expression in pleural metastatic cells could represent a major actor in the development of metastasis, associated with EMT and could account for a pejorative factor correlated with a poor Performance status.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center