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PLoS One. 2017 Aug 14;12(8):e0183136. doi: 10.1371/journal.pone.0183136. eCollection 2017.

Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.

Author information

1
Aix Marseille Univ, INSERM, GMGF, Marseille, France.
2
APHM, Hôpital la Timone, Service de Biologie Cellulaire, Marseille, France.
3
APHM, Hôpital Nord, Department of Thoracic Oncology-Pleural diseases-Interventional pulmonology, Marseille, France.
4
APHM, Hôpital la Timone, Département de Génétique Médicale et Centre de Ressources Biologiques, Marseille, France.
5
APHM, Hôpital la Timone, Service de Pharmacologie Clinique & Centre d'Investigation Clinique-CPCET, Marseille, France.
6
Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France.
7
Aix Marseille Univ, APHM, Marseille Early Phases Cancer Trials Center CLIP2, Marseille, France.
8
Aix Marseille Univ, Marseille, France.

Abstract

The type V intermediate filament lamins are the principal components of the nuclear matrix, including the nuclear lamina. Lamins are divided into A-type and B-type, which are encoded by three genes, LMNA, LMNB1, and LMNB2. The alternative splicing of LMNA produces two major A-type lamins, lamin A and lamin C. Previous studies have suggested that lamins are involved in cancer development and progression. A-type lamins have been proposed as biomarkers for cancer diagnosis, prognosis, and/or follow-up. The aim of the present study was to investigate lamins in cancer cells from metastatic pleural effusions using immunofluorescence, western blotting, and flow cytometry. In a sub-group of lung adenocarcinomas, we found reduced expression of lamin A but not of lamin C. The reduction in lamin A expression was correlated with the loss of epithelial membrane antigen (EMA)/MUC-1, an epithelial marker that is involved in the epithelial to mesenchymal transition (EMT). Finally, the lamin A expression was inversely correlated with the number of metastatic sites and the WHO Performance status, and association of pleural, bone and lung metastatic localizations was more frequent when lamin A expression was reduced. In conclusion, low lamin A but not lamin C expression in pleural metastatic cells could represent a major actor in the development of metastasis, associated with EMT and could account for a pejorative factor correlated with a poor Performance status.

PMID:
28806747
PMCID:
PMC5555706
DOI:
10.1371/journal.pone.0183136
[Indexed for MEDLINE]
Free PMC Article

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