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J Clin Invest. 2019 Jan 22. pii: 123931. doi: 10.1172/JCI123931. [Epub ahead of print]

Viral-mediated delivery of antibody targeting TAR DNA-binding protein 43 mitigates associated neuropathology.

Abstract

The cytoplasmic aggregation of TDP-43 is a hallmark of degenerating neurons in amyotrophic lateral sclerosis (ALS) and subsets of frontotemporal dementia (FTD). In order to reduce TDP-43 pathology, we have generated single chain (scFv) antibodies against the RNA recognition motif 1 (RRM1) of TDP-43 which is involved in abnormal protein self-aggregation and interaction with p65 nuclear factor kappa B (NFKB). Viral-mediated delivery into the nervous system of a scFv antibody, named VH7Vk9, reduced microgliosis in a mouse model of acute neuroinflammation and it mitigated cognitive impairment, motor defects, TDP-43 proteinopathy and neuroinflammation in transgenic mice expressing ALS-linked TDP-43 mutations. These results suggest that antibodies targeting the RRM1 domain of TDP-43 might provide new therapeutic avenues for treatment of ALS and FTD.

KEYWORDS:

Immunotherapy; Neurological disorders; Neuroscience; Therapeutics

PMID:
30667370
DOI:
10.1172/JCI123931
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