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Appl Environ Microbiol. 2018 Apr 2;84(8). pii: e02655-17. doi: 10.1128/AEM.02655-17. Print 2018 Apr 15.

Transferable Plasmid-Borne mcr-1 in a Colistin-Resistant Shigella flexneri Isolate.

Author information

1
Academy of Military Medical Sciences, Academy of Military Sciences, PLA, Beijing, China.
2
Institute of Disease Control and Prevention, PLA, Beijing, China.
3
Department of Parasitology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
4
Research Centre for Drugs and Diagnostics, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
5
Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
6
Beijing igeneCode Biotech Co., Ltd., Beijing, China.
7
Academy of Military Medical Sciences, Academy of Military Sciences, PLA, Beijing, China qiushf0613@hotmail.com hongbinsong@263.net.

Abstract

Since the initial discovery of mcr-1 in an Escherichia coli isolate from China, the gene has also been detected in Klebsiella pneumoniae and Salmonella enterica but is rarely reported in other Enterobacteriaceae Here, we report the isolation and identification of a Shigella flexneri strain harboring mcr-1 from stool samples in a pig farm in China from 2009. The MIC of colistin for the isolate is 4 μg/ml. Conjugation assays showed that the donor S. flexneri strain has functional and transferable colistin resistance. Sequencing revealed that mcr-1 was present on a putative composite transposon flanked by inverted repeats of ISApl1IMPORTANCE There are four species of Shigella, and Shigella flexneri is the most frequently isolated species in low- and middle-income countries (LMICs). In this study, we report a functional, transferable, plasmid-mediated mcr-1 gene in S. flexneri We have shown that mcr-1 is located on a novel composite transposon which is flanked by inverted repeats of ISApl1 The host strain is multidrug resistant, and this multidrug resistance is also transferable. The finding of a functional mcr-1 gene in S. flexneri, a human-associated Enterobacteriaceae family member, is a cause for concern as infections due to S. flexneri are the main Shigella infections in most low- and middle-income countries.

KEYWORDS:

ISApl1; Tn6390; composite transposon; multidrug resistance; plasmid transfer

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