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Sci Adv. 2016 Jan 29;2(1):e1500989. doi: 10.1126/sciadv.1500989. eCollection 2016 Jan.

An epidermis-driven mechanism positions and scales stem cell niches in plants.

Author information

1
Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.
2
Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.; INRA-CNRS-ENS Lyon-UCB Lyon 1, Laboratoire de Reproduction et Développement des Plantes, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.
3
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
4
INRA-CNRS-ENS Lyon-UCB Lyon 1, Laboratoire de Reproduction et Développement des Plantes, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.
5
Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.; Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.; Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA.
6
Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.; Computational Biology and Biological Physics Group, Department of Astronomy and Theoretical Physics, Lund University, S-221 00 Lund, Sweden.

Abstract

How molecular patterning scales to organ size is highly debated in developmental biology. We explore this question for the characteristic gene expression domains of the plant stem cell niche residing in the shoot apical meristem. We show that a combination of signals originating from the epidermal cell layer can correctly pattern the key gene expression domains and notably leads to adaptive scaling of these domains to the size of the tissue. Using live imaging, we experimentally confirm this prediction. The identified mechanism is also sufficient to explain de novo stem cell niches in emerging flowers. Our findings suggest that the deformation of the tissue transposes meristem geometry into an instructive scaling and positional input for the apical plant stem cell niche.

KEYWORDS:

Arabidopsis thaliana; CLAVATA3; Computational Morphodynamics; Shoot apical meristem; Stem cells; WUSCHEL

PMID:
27152324
PMCID:
PMC4846443
DOI:
10.1126/sciadv.1500989
[Indexed for MEDLINE]
Free PMC Article

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