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Microcirculation. 2019 Jun 27:e12579. doi: 10.1111/micc.12579. [Epub ahead of print]

Discovery of a bone-like blood particle in the peripheral circulation of humans and rodents.

Author information

1
Department of Kinesiology, University of Texas at Arlington, Arlington, Texas.
2
Delaware Biotechnology Institute, University of Delaware, Newark, Delaware.
3
Center for Translational Cancer Research, Helen F. Graham Cancer Center and Research Institute at Christiana Care Health System, Newark, Delaware.
4
Department of Medical and Molecular Sciences, University of Delaware, Newark, Delaware.
5
Flow Cytometry & LCM Core Facility, University of North Texas Health Science Center, Fort Worth, Texas.
6
Department of Kinesiology and Applied Physiology, University of Delaware, Newark, Delaware.

Abstract

OBJECTIVE:

To characterize ossified bone marrow blood vessels and confirm the presence of ossified particles (OSP) in humans and rodents.

METHODS:

Human bone marrow blood vessels were processed for scanning and transmission electron microscopy. Whole blood samples were collected from younger (26-39 years; n = 6) and older (55-63 years; n = 6) volunteers and male Fischer-344 rats (1 month, n = 7; 6 months, n = 7; 12 months, n = 7; 18-months, n = 6; 24 months, n = 8). OSP in the whole blood samples were sorted and imaged with microscopy to determine diameter, circularity, and solidity. Additionally, the chemical composition of OSP was determined via elemental analysis.

RESULTS:

SEM revealed two types of ossified bone marrow blood vessels: that is, "transitioning" and "ossified." OSP were adhered to the surface of transitioning vessels and theoretically gain access to and circulate within the blood. The majority of OSP were ≤15 μm in diameter, but many were of sufficient size to serve as emboli (ie, >15 μm).OSP were predominately oblong in shape and several had jagged tips and edges.

CONCLUSIONS:

We introduce a novel, bone-like blood particle that may be diagnostic of bone marrow blood vessel ossification. Further, OSP may associate with several disease states (eg, atherosclerosis).

KEYWORDS:

bone marrow blood vessel ossification; emboli; human; ossified particles; rat

PMID:
31246334
DOI:
10.1111/micc.12579

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