Format

Send to

Choose Destination
FEBS J. 2018 May;285(10):1827-1839. doi: 10.1111/febs.14449. Epub 2018 Apr 16.

Cysteine dioxygenase is essential for mouse sperm osmoadaptation and male fertility.

Author information

1
Faculty of Life and Environmental Sciences, University of Tsukuba, Japan.
2
The Baker Institute for Animal Health, Cornell University, Ithaca, NY, USA.
3
Department of Nutritional Sciences, Cornell University, Ithaca, NY, USA.
4
Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan.

Abstract

Sperm entering the epididymis are immotile and cannot respond to stimuli that will enable them to fertilize. The epididymis is a highly complex organ, with multiple histological zones and cell types that together change the composition and functional abilities of sperm through poorly understood mechanisms. Sperm take up taurine during epididymal transit, which may play antioxidant or osmoregulatory roles. Cysteine dioxygenase (CDO) is a critical enzyme for taurine synthesis. A previous study reported that male CDO-/- mice exhibit idiopathic infertility, prompting us to investigate the functions of CDO in male fertility. Immunoblotting and quantitative reverse transcription-polymerase chain reaction analysis of epididymal segments showed that androgen-dependent CDO expression was highest in the caput epididymidis. CDO-/- mouse sperm demonstrated a severe lack of in vitro fertilization ability. Acrosome exocytosis and tyrosine phosphorylation profiles in response to stimuli were normal, suggesting normal functioning of pathways associated with capacitation. CDO-/- sperm had a slight increase in head abnormalities. Taurine and hypotaurine concentrations in CDO-/- sperm decreased in the epididymal intraluminal fluid and sperm cytosol. We found no evidence of antioxidant protection against lipid peroxidation. However, CDO-/- sperm exhibited severe defects in volume regulation, swelling in response to the relatively hypo-osmotic conditions found in the female reproductive tract. Our findings suggest that epididymal CDO plays a key role in post-testicular sperm maturation, enabling sperm to osmoregulate as they transition from the male to the female reproductive tract, and provide new understanding of the compartmentalized functions of the epididymis.

KEYWORDS:

epididymal maturation; fertilization; sperm; taurine

PMID:
29604178
PMCID:
PMC5992081
[Available on 2019-05-01]
DOI:
10.1111/febs.14449

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center