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J Forensic Sci. 2018 Nov;63(6):1836-1840. doi: 10.1111/1556-4029.13757. Epub 2018 Feb 21.

A Comparison Among Three Multiplex Y-STR Profiling Kits for Sexual Assault Cases.

Author information

1
Department of Public Health, Forensic Sciences and Medical Education, Faculty of Medicine, University of Porto, Porto, Portugal.
2
National Institute of Legal Medicine and Forensic Sciences, Porto, Portugal.
3
Abel Salazar Biomedical Sciences Institute (ICBAS), University of Porto, Porto, Portugal.
4
CIIMAR, Matosinhos, Portugal.
5
Institute of Research and Advanced Training in Health Sciences and Technologies (IINFACTS), Department of Sciences, University Institute of Health Sciences (IUCS-CESPU), Gandra, Portugal.

Abstract

Biological evidence of sexual assault is one of the most difficult sample types to analyze in forensic laboratories. Y-STR markers are thus a valuable tool for analyzing these samples. The aim of this project was to compare three Y-STR commercial kits by analyzing their amplification performance on casework samples. Overall, 247 trace samples were analyzed with a Yfiler® Plus PCR Amplification Kit (Applied Biosystems), PowerPlex® Y23 (Promega® ) System and AmpFLSTR® Yfiler™ PCR Amplification Kit (Applied Biosystems). Comparing the amplification performance of the three kits, the first two were significantly more sensitive than the latter (p < 0.001). For samples, with a male DNA quantity less than 0.5 ng, the PowerPlex Y23® kit was the most sensitive and best performing kit, followed by the Yfiler® Plus kit (p = 0.009). In conclusion, the Yfiler® Plus and PowerPlex Y-23® kits are viable alternatives to older kits for samples with low amounts of male DNA.

KEYWORDS:

DNA typing; PowerPlex® Y23; Yfiler® Plus; amplification kits; forensic genetics; forensic science; sexual assault; short tandem repeats of Y chromosome

PMID:
29464703
DOI:
10.1111/1556-4029.13757
[Indexed for MEDLINE]

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