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Nanoscale. 2018 Jan 18;10(3):881-906. doi: 10.1039/c7nr08360b.

Technical challenges of working with extracellular vesicles.

Author information

1
Fundação Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil and Universidade Federal do Paraná, Curitiba, PR, Brazil.
2
CIPE, A.C.Camargo Cancer Center, São Paulo, SP, Brazil. emmanuel@cipe.accamargo.org.br.
3
School of Life Sciences, University of Nottingham, Nottingham, UK.
4
School of Life and Health Sciences, Aston University, England, UK.
5
University of Southampton, Southampton, England, UK.
6
Universidade de São Paulo, São Paulo, SP, Brazil.
7
Universidade de São Paulo, São Paulo, SP, Brazil and University of Gothenburg, Sweden.
8
Division of Cardiovascular Medicine, University of Oxford, Oxford, England, UK.
9
Acute Stroke Programme, RDM-Investigative Medicine, University of Oxford, Oxford, England, UK.
10
Sir William Dunn School of Pathology, University of Oxford, Oxford, England, UK.
11
University of St Andrews, St Andrews, Fife, Scotland, UK.
12
Federal University of São Paulo campus Diadema, Diadema, Brazil.
13
London Metropolitan University, London, England, UK.
14
Universidade Anhanguera de São Paulo, São Paulo, Brazil.
15
Universidade de São Paulo, São Paulo, SP, Brazil and University of Southern Denmark, Odense, Denmark.
16
Technische Universität München, Munich, Germany.
17
Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, Scotland, UK.
18
Oxford Brookes University, Oxford, England, UK.
19
Universidade de São Paulo, São Paulo, SP, Brazil and IRCCS, Fondazione Santa Lucia, Rome, Italy.
20
CIPE, A.C.Camargo Cancer Center, São Paulo, SP, Brazil. emmanuel@cipe.accamargo.org.br and Universidade de São Paulo, São Paulo, SP, Brazil.

Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

PMID:
29265147
DOI:
10.1039/c7nr08360b
[Indexed for MEDLINE]

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