Format

Send to

Choose Destination
Sci Rep. 2019 Apr 23;9(1):6466. doi: 10.1038/s41598-019-42923-2.

Optical intrinsic signal imaging with optogenetics reveals functional cortico-cortical connectivity at the columnar level in living macaques.

Author information

1
Lab for Integrative Neural Systems, RIKEN Center for Brain Science, Wako, Saitama, 351-0198, Japan.
2
Department of Complexity Science and Engineering, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8561, Japan.
3
Department of Neuroanatomy and Embryology, Fukushima Medical University, Fukushima, Fukushima, 960-1295, Japan.
4
Lab for Integrative Neural Systems, RIKEN Center for Brain Science, Wako, Saitama, 351-0198, Japan. tanifuji@riken.jp.
5
Department of Complexity Science and Engineering, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8561, Japan. tanifuji@riken.jp.
6
Department of Life Science and Medicine, Bio-Science Faculty of Science and Engineering, Waseda University, Shinjuku, Tokyo, 169-8555, Japan. tanifuji@riken.jp.

Abstract

Despite extensive research on primate cognitive function, understanding how anatomical connectivity at a neural circuit level relates to information transformation across different cortical areas remains primitive. New technology is needed to visualize inter-areal anatomical connectivity in living monkeys and to tie this directly to neurophysiological function. Here, we developed a novel method to investigate this structure-function relationship, by combining optical intrinsic signal imaging (OISI) with optogenetic stimulation in living monkeys (opto-OISI). The method involves expressing channelrhodophsin-2 in one area (source) followed by optical imaging of optogenetic activations in the other area (target). We successfully demonstrated the potential of the method with interhemispheric columnar projection patterns between V1/V2 border regions. Unlike the combination of optogenetics and functional magnetic resonance imaging (opto-fMRI), opto-OISI has the advantage of enabling us to detect responses of small clusters of neurons, even if the clusters are sparsely distributed. We suggest that opto-OISI can be a powerful approach to understanding cognitive function at the neural circuit level, directly linking inter-areal circuitry to fine-scale structure and function.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center