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Sci Rep. 2018 Sep 19;8(1):14046. doi: 10.1038/s41598-018-32386-2.

Proteomics study of human cord blood reticulocyte-derived exosomes.

Author information

1
ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
2
Instituto Aggeu Magalhães-FIOCRUZ, Recife, Pernambuco, Brazil.
3
IrsiCaixa AIDS Research Institute, Badalona, Spain.
4
Department of Pathology & REMAR-IVECAT Group, Hospital Universitari and Health Sciences Research Institute Germans Trias i Pujol, Universitat Autònoma de Barcelona, Barcelona, Spain.
5
IGTP Institut d'Investigació Germans Trias i Pujol, Badalona, Spain.
6
Universitat de Vic - Universitat Central de Catalunya (UVic-UCC), Vic, Spain.
7
Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain.
8
ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain. carmen.fernandez@isglobal.org.
9
IGTP Institut d'Investigació Germans Trias i Pujol, Badalona, Spain. carmen.fernandez@isglobal.org.
10
ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain. hernandoa.delportillo@isglobal.org.
11
IGTP Institut d'Investigació Germans Trias i Pujol, Badalona, Spain. hernandoa.delportillo@isglobal.org.
12
Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. hernandoa.delportillo@isglobal.org.

Abstract

Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.

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