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Nat Methods. 2019 Apr 29. doi: 10.1038/s41592-019-0393-z. [Epub ahead of print]

Instantaneous isotropic volumetric imaging of fast biological processes.

Author information

1
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
2
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
3
Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
4
Department of Pediatric Cardiology, University Hospital Heidelberg, Heidelberg, Germany.
5
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
6
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany. hufnagel@embl.de.
7
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany. prevedel@embl.de.
8
Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. prevedel@embl.de.
9
Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory, Monterotondo, Italy. prevedel@embl.de.

Abstract

To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.

PMID:
31036959
DOI:
10.1038/s41592-019-0393-z

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