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Nat Chem Biol. 2019 Mar;15(3):217-220. doi: 10.1038/s41589-018-0186-1. Epub 2018 Dec 10.

Riboregulated toehold-gated gRNA for programmable CRISPR-Cas9 function.

Author information

1
Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
2
Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA. wilfred@udel.edu.

Abstract

Predictable control over gene expression is essential to elicit desired synthetic cellular phenotypes. Although CRISPR-Cas9 offers a simple RNA-guided method for targeted transcriptional control, it lacks the ability to integrate endogenous cellular information for efficient signal processing. Here, we present a new class of riboregulators termed toehold-gated gRNA (thgRNA) by integrating toehold riboswitches into sgRNA scaffolds, and demonstrate their programmability for multiplexed regulation in Escherichia coli with minimal cross-talks.

PMID:
30531984
DOI:
10.1038/s41589-018-0186-1

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