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Nat Biotechnol. 2019 May;37(5):527-530. doi: 10.1038/s41587-019-0070-x. Epub 2019 Apr 1.

Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model.

Author information

1
Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium. Vikram.Virdi@vib-ugent.be.
2
VIB Center for Medical Biotechnology, Gent, Belgium. Vikram.Virdi@vib-ugent.be.
3
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium. Vikram.Virdi@vib-ugent.be.
4
VIB Center for Plant Systems Biology, Gent, Belgium. Vikram.Virdi@vib-ugent.be.
5
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium.
6
VIB Center for Plant Systems Biology, Gent, Belgium.
7
Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium.
8
VIB Center for Medical Biotechnology, Gent, Belgium.
9
Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
10
AVEVE Biochem, AVEVE Group, Leuven, Belgium.
11
Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium. Ann.Depicker@psb.vib-ugent.be.
12
VIB Center for Plant Systems Biology, Gent, Belgium. Ann.Depicker@psb.vib-ugent.be.
13
Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium. Nico.Callewaert@vib-ugent.be.
14
VIB Center for Medical Biotechnology, Gent, Belgium. Nico.Callewaert@vib-ugent.be.

Abstract

Oral antibodies that interfere with gastrointestinal targets and can be manufactured at scale are needed. Here we show that a single-gene-encoded monomeric immunoglobulin A (IgA)-like antibody, composed of camelid variable single domain antibodies (VHH) fused to IgA Fc (mVHH-IgA), prevents infection by enterotoxigenic Escherichia coli (F4-ETEC) in piglets. The mVHH-IgA can be produced in soybean seeds or secreted from the yeast Pichia pastoris, freeze- or spray-dried and orally delivered within food.

PMID:
30936561
DOI:
10.1038/s41587-019-0070-x

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