Send to

Choose Destination
Nat Microbiol. 2019 Apr;4(4):693-700. doi: 10.1038/s41564-018-0338-9. Epub 2019 Jan 28.

Megaphages infect Prevotella and variants are widespread in gut microbiomes.

Author information

Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA, USA.
Institute of Structural & Molecular Biology, Division of Biosciences, University College London, London, UK.
Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA.
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.
National Food Institute, Technical University of Denmark, Lyngby, Kongens, Denmark.
Department of Evolutionary Anthropology, Duke University, Durham, NC, USA.
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.
Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, USA.
Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA, USA.
The University of Melbourne, Melbourne, Victoria, Australia.
Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.


Bacteriophages (phages) dramatically shape microbial community composition, redistribute nutrients via host lysis and drive evolution through horizontal gene transfer. Despite their importance, much remains to be learned about phages in the human microbiome. We investigated the gut microbiomes of humans from Bangladesh and Tanzania, two African baboon social groups and Danish pigs; many of these microbiomes contain phages belonging to a clade with genomes‚ÄČ>540‚ÄČkilobases in length, the largest yet reported in the human microbiome and close to the maximum size ever reported for phages. We refer to these as Lak phages. CRISPR spacer targeting indicates that Lak phages infect bacteria of the genus Prevotella. We manually curated to completion 15 distinct Lak phage genomes recovered from metagenomes. The genomes display several interesting features, including use of an alternative genetic code, large intergenic regions that are highly expressed and up to 35 putative transfer RNAs, some of which contain enigmatic introns. Different individuals have distinct phage genotypes, and shifts in variant frequencies over consecutive sampling days reflect changes in the relative abundance of phage subpopulations. Recent homologous recombination has resulted in extensive genome admixture of nine baboon Lak phage populations. We infer that Lak phages are widespread in gut communities that contain the Prevotella species, and conclude that megaphages, with fascinating and underexplored biology, may be common but largely overlooked components of human and animal gut microbiomes.

Comment in


Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center