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Nat Commun. 2019 Mar 13;10(1):1191. doi: 10.1038/s41467-019-09081-5.

Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity.

Author information

1
Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Forschungszentrum Jülich GmbH, Jülich, 52425, Germany.
2
Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, 85764, Germany.
3
Institute of Bio- and Geosciences (IBG-1): Biotechnology, Forschungszentrum Jülich GmbH, Jülich, 52425, Germany.
4
Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Forschungszentrum Jülich GmbH, Jülich, 52425, Germany. t.drepper@fz-juelich.de.
5
Chair of Biological Imaging and Center for Translational Cancer Research (TranslaTUM), Technische Universität München, München, 81675, Germany.
6
Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, 85764, Germany. andre.stiel@helmholtz-muenchen.de.

Abstract

Τhe morphology, physiology and immunology, of solid tumors exhibit spatial heterogeneity which complicates our understanding of cancer progression and therapy response. Understanding spatial heterogeneity necessitates high resolution in vivo imaging of anatomical and pathophysiological tumor information. We introduce Rhodobacter as bacterial reporter for multispectral optoacoustic (photoacoustic) tomography (MSOT). We show that endogenous bacteriochlorophyll a in Rhodobacter gives rise to strong optoacoustic signals >800 nm away from interfering endogenous absorbers. Importantly, our results suggest that changes in the spectral signature of Rhodobacter which depend on macrophage activity inside the tumor can be used to reveal heterogeneity of the tumor microenvironment. Employing non-invasive high resolution MSOT in longitudinal studies we show spatiotemporal changes of Rhodobacter spectral profiles in mice bearing 4T1 and CT26.WT tumor models. Accessibility of Rhodobacter to genetic modification and thus to sensory and therapeutic functions suggests potential for a theranostic platform organism.

PMID:
30867430
PMCID:
PMC6416252
DOI:
10.1038/s41467-019-09081-5
[Indexed for MEDLINE]
Free PMC Article

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