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Nat Commun. 2019 Mar 5;10(1):1051. doi: 10.1038/s41467-019-08973-w.

The Wolbachia mobilome in Culex pipiens includes a putative plasmid.

Author information

1
ASTRE, INRA, CIRAD, University of Montpellier, Montpellier, 34398, France. reveillaud.j@gmail.com.
2
Department of Biological Sciences, Vanderbilt University, Nashville, 37235, TN, USA.
3
Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut de Biologie François Jacob, Genoscope, Evry, 91057, France.
4
Graduate Program in the Biophysical Sciences, University of Chicago, Chicago, IL, 60637, USA.
5
Department of Medicine, University of Chicago, Chicago, 60637, IL, USA.
6
Institut des Sciences de l'Evolution de Montpellier (ISEM), UMR CNRS-IRD-EPHE-Université de Montpellier, Montpellier, 34095, France.
7
ASTRE, INRA, CIRAD, University of Montpellier, Montpellier, 34398, France.
8
Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, 37235, TN, USA.
9
Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University, Nashville, 37235, TN, USA.
10
Graduate Program in the Biophysical Sciences, University of Chicago, Chicago, IL, 60637, USA. a.murat.eren@gmail.com.
11
Department of Medicine, University of Chicago, Chicago, 60637, IL, USA. a.murat.eren@gmail.com.
12
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, 02543, MA, USA. a.murat.eren@gmail.com.

Abstract

Wolbachia is a genus of obligate intracellular bacteria found in nematodes and arthropods worldwide, including insect vectors that transmit dengue, West Nile, and Zika viruses. Wolbachia's unique ability to alter host reproductive behavior through its temperate bacteriophage WO has enabled the development of new vector control strategies. However, our understanding of Wolbachia's mobilome beyond its bacteriophages is incomplete. Here, we reconstruct near-complete Wolbachia genomes from individual ovary metagenomes of four wild Culex pipiens mosquitoes captured in France. In addition to viral genes missing from the Wolbachia reference genome, we identify a putative plasmid (pWCP), consisting of a 9.23-kbp circular element with 14 genes. We validate its presence in additional Culex pipiens mosquitoes using PCR, long-read sequencing, and screening of existing metagenomes. The discovery of this previously unrecognized extrachromosomal element opens additional possibilities for genetic manipulation of Wolbachia.

PMID:
30837458
PMCID:
PMC6401122
DOI:
10.1038/s41467-019-08973-w
[Indexed for MEDLINE]
Free PMC Article

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