Format

Send to

Choose Destination
Nat Commun. 2019 Mar 5;10(1):895. doi: 10.1038/s41467-019-08806-w.

Sleep increases chromosome dynamics to enable reduction of accumulating DNA damage in single neurons.

Author information

1
The Faculty of Life Sciences and the Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
2
Department of Physics and the Institute for Nanotechnology, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
3
The Faculty of Life Sciences and the Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat-Gan, 5290002, Israel. lior.appelbaum@biu.ac.il.

Abstract

Sleep is essential to all animals with a nervous system. Nevertheless, the core cellular function of sleep is unknown, and there is no conserved molecular marker to define sleep across phylogeny. Time-lapse imaging of chromosomal markers in single cells of live zebrafish revealed that sleep increases chromosome dynamics in individual neurons but not in two other cell types. Manipulation of sleep, chromosome dynamics, neuronal activity, and DNA double-strand breaks (DSBs) showed that chromosome dynamics are low and the number of DSBs accumulates during wakefulness. In turn, sleep increases chromosome dynamics, which are necessary to reduce the amount of DSBs. These results establish chromosome dynamics as a potential marker to define single sleeping cells, and propose that the restorative function of sleep is nuclear maintenance.

PMID:
30837464
PMCID:
PMC6401120
DOI:
10.1038/s41467-019-08806-w
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center