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Nat Commun. 2019 Jan 22;10(1):371. doi: 10.1038/s41467-019-08312-z.

Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes.

Author information

1
Institute for Genome Sciences, University of Maryland School of Medicine, 670 W Baltimore Street, Baltimore, MD, 21201, USA.
2
Malaria Molecular Epidemiology Unit, Institut Pasteur in Cambodia, 5 Boulevard Monivong, PO Box 983, Phnom Penh, 12 201, Cambodia.
3
Biology of Host-Parasite Interactions Unit, Institut Pasteur, 25-28 Rue du Dr. Roux, 75724, Paris, France.
4
Institute for Genome Sciences, University of Maryland School of Medicine, 670 W Baltimore Street, Baltimore, MD, 21201, USA. dserre@som.umaryland.edu.

Abstract

Studies of Plasmodium vivax gene expression are complicated by the lack of in vitro culture system and the difficulties associated with studying clinical infections that often contain multiple clones and a mixture of parasite stages. Here, we characterize the transcriptomes of P. vivax parasites from 26 malaria patients. We show that most parasite mRNAs derive from trophozoites and that the asynchronicity of P. vivax infections is therefore unlikely to confound gene expression studies. Analyses of gametocyte genes reveal two distinct clusters of co-regulated genes, suggesting that male and female gametocytes are independently regulated. Finally, we analyze gene expression changes induced by chloroquine and show that this antimalarial drug efficiently eliminates most P. vivax parasite stages but, in contrast to P. falciparum, does not affect trophozoites.

PMID:
30670687
PMCID:
PMC6342968
DOI:
10.1038/s41467-019-08312-z
[Indexed for MEDLINE]
Free PMC Article

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