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Nat Commun. 2019 Jan 29;10(1):481. doi: 10.1038/s41467-018-08261-z.

Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion.

Author information

1
Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, M5G 2C4, ON, Canada.
2
Toronto Centre for Liver Disease, University Health Network, Toronto General Hospital, Toronto, M5G 2C4, ON, Canada. Jordan.feld@uhn.ca.
3
São Carlos Institute of Physics, University of São Paulo Brazil, São Paulo, 13566-590, Brazil.
4
Toronto Centre for Liver Disease, University Health Network, Toronto General Hospital, Toronto, M5G 2C4, ON, Canada.
5
Princess Margaret Cancer Centre/Department of Medical Biophysics, University of Toronto, Toronto, M5G 2C4, Canada.
6
Multi-Organ Transplant Program, University Health Network, Toronto, M5G 2C4, ON, Canada.
7
Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, M5G 2C4, ON, Canada. marcelo.cypel@uhn.ca.
8
Multi-Organ Transplant Program, University Health Network, Toronto, M5G 2C4, ON, Canada. marcelo.cypel@uhn.ca.

Abstract

Availability of organs is a limiting factor for lung transplantation, leading to substantial mortality rates on the wait list. Use of organs from donors with transmissible viral infections, such as hepatitis C virus (HCV), would increase organ donation, but these organs are generally not offered for transplantation due to a high risk of transmission. Here, we develop a method for treatment of HCV-infected human donor lungs that prevents HCV transmission. Physical viral clearance in combination with germicidal light-based therapies during normothermic ex-vivo Lung Perfusion (EVLP), a method for assessment and treatment of injured donor lungs, inactivates HCV virus in a short period of time. Such treatment is shown to be safe using a large animal EVLP-to-lung transplantation model. This strategy of treating viral infection in a donor organ during preservation could significantly increase the availability of organs for transplantation and encourages further clinical development.

PMID:
30696822
PMCID:
PMC6351537
DOI:
10.1038/s41467-018-08261-z
[Indexed for MEDLINE]
Free PMC Article

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